Once the basic design for a shape has been completed, one can add a surface pattern on top of the shape. I maintain a list of researchers in DNA origami, as well as lists and repositories of references which I am happy to share. If you are interested in DNA origami, and DNA nanotechnology in general, and want more information, please email me. The latest version of caDNAno is recommended, but you may also be interested in a previous version, available from a repository of legacy caDNAno code. Thus it has really become the standard for DNA origami design. It features a great graphical user interface, and a large number of other scientists are developing software that interfaces with caDNAno. We use caDNAno to design DNA origami almost every day. Luckily it was quickly obsolesced by much better software caDNAno, by Shawn Douglas at Harvard. Unfortunately the code was in such a bad state that it proved too difficult to clean up for release. I apologize that the MATLAB code for the design of origami structures has not been released. Please email me with your PDF viewer name and version if either of these files do not work for you. It cannot be printed clearly and is best viewed with a PDF viewer on the screen. This file is useful if one wishes to check details of the design or modify them. The second ( 9 pages, 192 kilobytes) includes diagrams for all the designs that have the full sequence written out in the diagrams. This file is likely to be of most interest. The first ( 82 pages, 6.3 megabytes) describes the design method, block diagrams for the designs, sequences for all of the designs, and includes data on control experiments. There are two supplemental files associated with this paper. The best way to understand how this works is to read the original article published in Nature. We hope to use the technique of DNA origami (as well as many other techniques of DNA nanotechnology) to build smaller, faster computers and many other devices. While the smiley face shape is somewhat silly DNA artwork, it is a high technology artifact and there is serious science behind it. M13 is a bacteriophage-it can make the bacteria in your intestine sick but not you! Also, I apologize to my Japanese colleagues for the name “DNA origami” which literally translated would be “DNA paper folding”-English speakers sometimes use “origami” as verb meaning just “to fold up”, similar to the way we use “pretzel” as a verb-thus “DNA origami” had the feeling of “DNA folding” even though it is an abuse of the word “origami”.) (For the record: there is no fundamental significance to the fact that it is viral DNA I could buy it and it was cheap and pure. I call the method “scaffolded DNA origami”. These short strands fold the long strand into the smiley face shape. The other 7000 of these bases belong to about 250 shorter strands, each about 30 bases long. 7000 of these DNA bases belong to a long single strand, a DNA molecule that just happens to be the genome of the virus M13. Each is about 100 nanometers across (1/1000th the width of a human hair), 2 nanometers thick, and each is comprised of about 14,000 DNA bases. Each of the two smiley faces above are actually giant DNA complexes imaged with an atomic force microscope. In 2006, I reported a method of creating nanoscale shapes and patterns using DNA.
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